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Purification and characterization of recombinant FAD synthetase from Neurosporacrassa

FAD Synthetase (FADS) [EC 2.7.7.2], the second enzyme in flavin cofactor biosynthetic pathway converts FMN to FAD, plays an important role in many redox reactions. Neurospora crassa FADS (NcFADS) was cloned and overexpressed in E. coli cells. Recombinant NcFADS was purified in high yields of ∼8 mg p...

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Detalles Bibliográficos
Autores principales: Puvvada, Nirupama, Gunde, Sridhar, Ramana Devi, Ch. Venkata, Gogada, Raghu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571487/
https://www.ncbi.nlm.nih.gov/pubmed/34765745
http://dx.doi.org/10.1016/j.bbrep.2021.101161