Biochemical approach for isolation of polyadenylated RNAs with bound proteins from yeast

In vivo characterization of RNA-protein interactions is the key for understanding RNA regulatory mechanisms. Herein, we describe a protocol for detection of proteins interacting with polyadenylated RNAs in the yeast Saccharomyces cerevisiae. Proteins are crosslinked to nucleic acids in vivo by ultra...

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Detalles Bibliográficos
Autores principales: Matia-González, Ana M., Jabre, Ibtissam, Gerber, André P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571520/
https://www.ncbi.nlm.nih.gov/pubmed/34766032
http://dx.doi.org/10.1016/j.xpro.2021.100929

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571520/
https://www.ncbi.nlm.nih.gov/pubmed/34766032
http://dx.doi.org/10.1016/j.xpro.2021.100929

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