A low-cost and open-source protocol to produce key enzymes for molecular detection assays

Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular detection of pathogens. Based on previous reports, we optimized the protocol for LbCas12a, Taq DNA polymerase, M-MLV reverse transcriptase, and...

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Detalles Bibliográficos
Autores principales: Mendoza-Rojas, Gabriel, Sarabia-Vega, Vanessa, Sanchez-Castro, Ana, Tello, Lesia, Cabrera-Sosa, Luis, Nakamoto, Jose A., Peñaranda, Katherin, Adaui, Vanessa, Alcántara, Roberto, Milón, Pohl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571801/
https://www.ncbi.nlm.nih.gov/pubmed/34766029
http://dx.doi.org/10.1016/j.xpro.2021.100899

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571801/
https://www.ncbi.nlm.nih.gov/pubmed/34766029
http://dx.doi.org/10.1016/j.xpro.2021.100899

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