Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells

BACKGROUND: Delivery of CRISPR/Cas RNPs to target cells still remains the biggest bottleneck to genome editing. Many efforts are made to develop efficient CRISPR/Cas RNP delivery methods that will not affect viability of target cell dramatically. Popular current methods and protocols of CRISPR/Cas R...

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Detalles Bibliográficos
Autores principales: Tyumentseva, Marina A., Tyumentsev, Aleksandr I., Akimkin, Vasiliy G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Study Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577758/
https://www.ncbi.nlm.nih.gov/pubmed/34752487
http://dx.doi.org/10.1371/journal.pone.0259812

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577758/
https://www.ncbi.nlm.nih.gov/pubmed/34752487
http://dx.doi.org/10.1371/journal.pone.0259812

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