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Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells
BACKGROUND: Delivery of CRISPR/Cas RNPs to target cells still remains the biggest bottleneck to genome editing. Many efforts are made to develop efficient CRISPR/Cas RNP delivery methods that will not affect viability of target cell dramatically. Popular current methods and protocols of CRISPR/Cas R...
Autores principales: | Tyumentseva, Marina A., Tyumentsev, Aleksandr I., Akimkin, Vasiliy G. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577758/ https://www.ncbi.nlm.nih.gov/pubmed/34752487 http://dx.doi.org/10.1371/journal.pone.0259812 |
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