CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens

Off-target effects are well established confounders of CRISPR negative selection screens that impair the identification of essential genomic loci. In particular, non-coding regulatory elements and repetitive regions are often difficult to target with specific gRNAs, effectively precluding the unbias...

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Detalles Bibliográficos
Autores principales: Perez, Alexendar R., Sala, Laura, Perez, Richard K., Vidigal, Joana A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578331/
https://www.ncbi.nlm.nih.gov/pubmed/34753924
http://dx.doi.org/10.1038/s41467-021-26722-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578331/
https://www.ncbi.nlm.nih.gov/pubmed/34753924
http://dx.doi.org/10.1038/s41467-021-26722-w

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