Cargando…

CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens

Off-target effects are well established confounders of CRISPR negative selection screens that impair the identification of essential genomic loci. In particular, non-coding regulatory elements and repetitive regions are often difficult to target with specific gRNAs, effectively precluding the unbias...

Descripción completa

Detalles Bibliográficos
Autores principales:	Perez, Alexendar R., Sala, Laura, Perez, Richard K., Vidigal, Joana A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578331/ https://www.ncbi.nlm.nih.gov/pubmed/34753924 http://dx.doi.org/10.1038/s41467-021-26722-w

Ejemplares similares

Author Correction: CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens
por: Perez, Alexendar R., et al.
Publicado: (2022)

Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries
por: Vidigal, Joana A., et al.
Publicado: (2015)

CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design
por: Anthon, Christian, et al.
Publicado: (2022)

Construction and evaluation of gRNA arrays for multiplex CRISPR‐Cas9
por: Žun, Gašper, et al.
Publicado: (2023)

The Current State and Future of CRISPR-Cas9 gRNA Design Tools
por: Wilson, Laurence O. W., et al.
Publicado: (2018)

gRNA validation for wheat genome editing with the CRISPR-Cas9 system
por: Arndell, Taj, et al.
Publicado: (2019)

Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis
por: Lee, Raymond Teck Ho, et al.
Publicado: (2016)

Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays
por: Kurata, Morito, et al.
Publicado: (2018)

GuideScan software for improved single and paired CRISPR guide RNA design
por: Perez, Alexendar R., et al.
Publicado: (2017)

Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression
por: Adikusuma, Fatwa, et al.
Publicado: (2017)

Drosophila balancer reengineering using polycistronic gRNA for CRISPR/Cas9 gene editing
por: Benner, Leif, et al.
Publicado: (2018)

Synthetic Circular gRNA Mediated Biological Function of CRISPR-(d)Cas9 System
por: Wang, Mingxia, et al.
Publicado: (2022)

Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning
por: Xiang, Xi, et al.
Publicado: (2021)

The gRNA-miRNA-gRNA Ternary Cassette Combining CRISPR/Cas9 with RNAi Approach Strongly Inhibits Hepatitis B Virus Replication
por: Wang, Jie, et al.
Publicado: (2017)

Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
por: Moreb, E. A., et al.
Publicado: (2021)

CRISPR–Cas9 gRNA efficiency prediction: an overview of predictive tools and the role of deep learning
por: Konstantakos, Vasileios, et al.
Publicado: (2022)

CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context
por: Corsi, Giulia I., et al.
Publicado: (2022)

In Planta Processing of the SpCas9–gRNA Complex
por: Mikami, Masafumi, et al.
Publicado: (2017)

Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging
por: Hong, Yu, et al.
Publicado: (2018)

Screening of CRISPR/Cas9 gRNA for mimicking Powdery Mildew resistant MLO ol-2 mutant
por: Prajapati, Archana, et al.
Publicado: (2021)

Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage
por: Riesenberg, Stephan, et al.
Publicado: (2022)

Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes
por: Allen, Sarah E., et al.
Publicado: (2021)

An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations
por: Lu, Zhike, et al.
Publicado: (2022)

Study of the effect of the introduction of mitochondrial import determinants into the gRNA structure on the activity of the gRNA/SpCas9 complex in vitro
por: Zakirova, E.G., et al.
Publicado: (2020)

A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism
por: Arakawa, Hiroshi
Publicado: (2016)

Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger
por: Song, Letian, et al.
Publicado: (2018)

Programmed gRNA Removal System for CRISPR-Cas9-Mediated Multi-Round Genome Editing in Bacillus subtilis
por: Lim, Hayeon, et al.
Publicado: (2019)

CRISPR-Cas9 Dual-gRNA Attack Causes Mutation, Excision and Inversion of the HIV-1 Proviral DNA
por: Binda, Caroline S., et al.
Publicado: (2020)

Synthetic gRNA/Cas9 Ribonucleoprotein Inhibits HIV Reactivation and Replication
por: Khanal, Sushant, et al.
Publicado: (2022)

Uncertainty-aware and interpretable evaluation of Cas9–gRNA and Cas12a–gRNA specificity for fully matched and partially mismatched targets with Deep Kernel Learning
por: Kirillov, Bogdan, et al.
Publicado: (2021)

CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency
por: Zhao, Dongdong, et al.
Publicado: (2017)

A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
por: Zhang, Yueping, et al.
Publicado: (2019)

Targeted delivery of CRISPR/Cas9 to prostate cancer by modified gRNA using a flexible aptamer-cationic liposome
por: Zhen, Shuai, et al.
Publicado: (2016)

5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage
por: Mullally, Grace, et al.
Publicado: (2020)

Structure-based design of gRNA for Cas13
por: Bandaru, Srinivas, et al.
Publicado: (2020)

gRNA Sequence Heterology Tolerance Catalyzed by CRISPR/Cas in an In Vitro Homology-Directed Repair Reaction
por: Hewes, Amanda M., et al.
Publicado: (2020)

Generation of multi-gene knockout rabbits using the Cas9/gRNA system
por: Yan, Quanmei, et al.
Publicado: (2014)

Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX
por: Yu, Xin, et al.
Publicado: (2016)

Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies
por: Jensen, Emil D., et al.
Publicado: (2017)

Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts
por: Yoo, Byung-Chun, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_0hmir96m3lp6nn2bsdqrc5fsl5