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Optimization of library preparation based on SMART for ultralow RNA-seq in mice brain tissues

BACKGROUND: Single-cell RNA sequencing (scRNA-seq) provides new insights to address biological and medical questions, and it will benefit more from the ultralow input RNA or subcellular sequencing. RESULTS: Here, we present a highly sensitive library construction protocol for ultralow input RNA sequ...

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Detalles Bibliográficos
Autores principales: Jia, Erteng, Shi, Huajuan, Wang, Ying, Zhou, Ying, Liu, Zhiyu, Pan, Min, Bai, Yunfei, Zhao, Xiangwei, Ge, Qinyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579666/
https://www.ncbi.nlm.nih.gov/pubmed/34758728
http://dx.doi.org/10.1186/s12864-021-08132-w