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Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets

The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands of mouse s...

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Detalles Bibliográficos
Autores principales: Ito, Daiyu, Wakayama, Teruhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581645/
https://www.ncbi.nlm.nih.gov/pubmed/34806046
http://dx.doi.org/10.1016/j.xpro.2021.100933