Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets

The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands of mouse s...

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Detalles Bibliográficos
Autores principales: Ito, Daiyu, Wakayama, Teruhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581645/
https://www.ncbi.nlm.nih.gov/pubmed/34806046
http://dx.doi.org/10.1016/j.xpro.2021.100933
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author Ito, Daiyu
Wakayama, Teruhiko
author_facet Ito, Daiyu
Wakayama, Teruhiko
author_sort Ito, Daiyu
collection PubMed
description The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands of mouse strains in a card folder. We can also send the FD sperm domestically using a postcard without any extra equipment. For complete details on the use and execution of this protocol, please refer to Ito et al. (2021).
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spelling pubmed-85816452021-11-18 Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets Ito, Daiyu Wakayama, Teruhiko STAR Protoc Protocol The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands of mouse strains in a card folder. We can also send the FD sperm domestically using a postcard without any extra equipment. For complete details on the use and execution of this protocol, please refer to Ito et al. (2021). Elsevier 2021-11-03 /pmc/articles/PMC8581645/ /pubmed/34806046 http://dx.doi.org/10.1016/j.xpro.2021.100933 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ito, Daiyu
Wakayama, Teruhiko
Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title_full Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title_fullStr Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title_full_unstemmed Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title_short Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title_sort protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581645/
https://www.ncbi.nlm.nih.gov/pubmed/34806046
http://dx.doi.org/10.1016/j.xpro.2021.100933
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