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Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands of mouse s...
Autores principales: | Ito, Daiyu, Wakayama, Teruhiko |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581645/ https://www.ncbi.nlm.nih.gov/pubmed/34806046 http://dx.doi.org/10.1016/j.xpro.2021.100933 |
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