Cargando…

RIF1 Links Replication Timing with Fork Reactivation and DNA Double-Strand Break Repair

Replication timing (RT) is a cellular program to coordinate initiation of DNA replication in all origins within the genome. RIF1 (replication timing regulatory factor 1) is a master regulator of RT in human cells. This role of RIF1 is associated with binding G4-quadruplexes and changes in 3D chromat...

Descripción completa

Detalles Bibliográficos
Autores principales: Blasiak, Janusz, Szczepańska, Joanna, Sobczuk, Anna, Fila, Michal, Pawlowska, Elzbieta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583789/
https://www.ncbi.nlm.nih.gov/pubmed/34768871
http://dx.doi.org/10.3390/ijms222111440
_version_ 1784597288217214976
author Blasiak, Janusz
Szczepańska, Joanna
Sobczuk, Anna
Fila, Michal
Pawlowska, Elzbieta
author_facet Blasiak, Janusz
Szczepańska, Joanna
Sobczuk, Anna
Fila, Michal
Pawlowska, Elzbieta
author_sort Blasiak, Janusz
collection PubMed
description Replication timing (RT) is a cellular program to coordinate initiation of DNA replication in all origins within the genome. RIF1 (replication timing regulatory factor 1) is a master regulator of RT in human cells. This role of RIF1 is associated with binding G4-quadruplexes and changes in 3D chromatin that may suppress origin activation over a long distance. Many effects of RIF1 in fork reactivation and DNA double-strand (DSB) repair (DSBR) are underlined by its interaction with TP53BP1 (tumor protein p53 binding protein). In G1, RIF1 acts antagonistically to BRCA1 (BRCA1 DNA repair associated), suppressing end resection and homologous recombination repair (HRR) and promoting non-homologous end joining (NHEJ), contributing to DSBR pathway choice. RIF1 is an important element of intra-S-checkpoints to recover damaged replication fork with the involvement of HRR. High-resolution microscopic studies show that RIF1 cooperates with TP53BP1 to preserve 3D structure and epigenetic markers of genomic loci disrupted by DSBs. Apart from TP53BP1, RIF1 interact with many other proteins, including proteins involved in DNA damage response, cell cycle regulation, and chromatin remodeling. As impaired RT, DSBR and fork reactivation are associated with genomic instability, a hallmark of malignant transformation, RIF1 has a diagnostic, prognostic, and therapeutic potential in cancer. Further studies may reveal other aspects of common regulation of RT, DSBR, and fork reactivation by RIF1.
format Online
Article
Text
id pubmed-8583789
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85837892021-11-12 RIF1 Links Replication Timing with Fork Reactivation and DNA Double-Strand Break Repair Blasiak, Janusz Szczepańska, Joanna Sobczuk, Anna Fila, Michal Pawlowska, Elzbieta Int J Mol Sci Review Replication timing (RT) is a cellular program to coordinate initiation of DNA replication in all origins within the genome. RIF1 (replication timing regulatory factor 1) is a master regulator of RT in human cells. This role of RIF1 is associated with binding G4-quadruplexes and changes in 3D chromatin that may suppress origin activation over a long distance. Many effects of RIF1 in fork reactivation and DNA double-strand (DSB) repair (DSBR) are underlined by its interaction with TP53BP1 (tumor protein p53 binding protein). In G1, RIF1 acts antagonistically to BRCA1 (BRCA1 DNA repair associated), suppressing end resection and homologous recombination repair (HRR) and promoting non-homologous end joining (NHEJ), contributing to DSBR pathway choice. RIF1 is an important element of intra-S-checkpoints to recover damaged replication fork with the involvement of HRR. High-resolution microscopic studies show that RIF1 cooperates with TP53BP1 to preserve 3D structure and epigenetic markers of genomic loci disrupted by DSBs. Apart from TP53BP1, RIF1 interact with many other proteins, including proteins involved in DNA damage response, cell cycle regulation, and chromatin remodeling. As impaired RT, DSBR and fork reactivation are associated with genomic instability, a hallmark of malignant transformation, RIF1 has a diagnostic, prognostic, and therapeutic potential in cancer. Further studies may reveal other aspects of common regulation of RT, DSBR, and fork reactivation by RIF1. MDPI 2021-10-23 /pmc/articles/PMC8583789/ /pubmed/34768871 http://dx.doi.org/10.3390/ijms222111440 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Blasiak, Janusz
Szczepańska, Joanna
Sobczuk, Anna
Fila, Michal
Pawlowska, Elzbieta
RIF1 Links Replication Timing with Fork Reactivation and DNA Double-Strand Break Repair
title RIF1 Links Replication Timing with Fork Reactivation and DNA Double-Strand Break Repair
title_full RIF1 Links Replication Timing with Fork Reactivation and DNA Double-Strand Break Repair
title_fullStr RIF1 Links Replication Timing with Fork Reactivation and DNA Double-Strand Break Repair
title_full_unstemmed RIF1 Links Replication Timing with Fork Reactivation and DNA Double-Strand Break Repair
title_short RIF1 Links Replication Timing with Fork Reactivation and DNA Double-Strand Break Repair
title_sort rif1 links replication timing with fork reactivation and dna double-strand break repair
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583789/
https://www.ncbi.nlm.nih.gov/pubmed/34768871
http://dx.doi.org/10.3390/ijms222111440
work_keys_str_mv AT blasiakjanusz rif1linksreplicationtimingwithforkreactivationanddnadoublestrandbreakrepair
AT szczepanskajoanna rif1linksreplicationtimingwithforkreactivationanddnadoublestrandbreakrepair
AT sobczukanna rif1linksreplicationtimingwithforkreactivationanddnadoublestrandbreakrepair
AT filamichal rif1linksreplicationtimingwithforkreactivationanddnadoublestrandbreakrepair
AT pawlowskaelzbieta rif1linksreplicationtimingwithforkreactivationanddnadoublestrandbreakrepair