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Protocol for design, construction, and selection of genome phage (gPhage) display libraries

This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape against th...

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Detalles Bibliográficos
Autores principales: Rodriguez Carnero, Luis Antonio, Reis Teixeira, André Azevedo, Fen Tang, Fenny Hui, Kuramoto, Andréia, Manso Alves, Maria Júlia, Colli, Walter, Setubal, João Carlos, Cunha-Neto, Edécio, Pasqualini, Renata, Arap, Wadih, Giordano, Ricardo José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590080/
https://www.ncbi.nlm.nih.gov/pubmed/34806047
http://dx.doi.org/10.1016/j.xpro.2021.100936