Protocol for design, construction, and selection of genome phage (gPhage) display libraries

This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape against th...

Descripción completa

Detalles Bibliográficos
Autores principales: Rodriguez Carnero, Luis Antonio, Reis Teixeira, André Azevedo, Fen Tang, Fenny Hui, Kuramoto, Andréia, Manso Alves, Maria Júlia, Colli, Walter, Setubal, João Carlos, Cunha-Neto, Edécio, Pasqualini, Renata, Arap, Wadih, Giordano, Ricardo José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590080/
https://www.ncbi.nlm.nih.gov/pubmed/34806047
http://dx.doi.org/10.1016/j.xpro.2021.100936
Descripción
Sumario:This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape against the parasite. Here, we used an organism with a relatively large but intronless genome, although future applications could include other prevalent or (re)emerging infectious organisms carrying genomes with a limited number of introns. For complete details on the use and execution of this protocol, please refer to Teixeira et al. (2021).

Ejemplares similares