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Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing

Nematodes are abundant metazoans that play crucial roles in nutrient recycle in the pedosphere. Although high-throughput amplicon sequencing is a powerful tool for the taxonomic profiling of soil nematodes, polymerase chain reaction (PCR) primers for amplification of the 18S ribosomal RNA (SSU) gene...

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Detalles Bibliográficos
Autores principales: Kenmotsu, Harutaro, Takabayashi, Emi, Takase, Akinori, Hirose, Yuu, Eki, Toshihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592498/
https://www.ncbi.nlm.nih.gov/pubmed/34780544
http://dx.doi.org/10.1371/journal.pone.0259842