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Immunoaffinity purification of endogenous proteins from S. cerevisiae for post-translational modification and protein interaction analysis
Protein regulation by post-translational modifications and protein-protein interactions is critical to controlling molecular pathways. Here, we describe an immunoaffinity purification approach in Saccharomyces cerevisiae. The protocol uses an endogenously-expressed epitope-tagged protein and can be...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593661/ https://www.ncbi.nlm.nih.gov/pubmed/34816128 http://dx.doi.org/10.1016/j.xpro.2021.100945 |