Immunoaffinity purification of endogenous proteins from S. cerevisiae for post-translational modification and protein interaction analysis

Protein regulation by post-translational modifications and protein-protein interactions is critical to controlling molecular pathways. Here, we describe an immunoaffinity purification approach in Saccharomyces cerevisiae. The protocol uses an endogenously-expressed epitope-tagged protein and can be...

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Detalles Bibliográficos
Autores principales: Jaiswal, Deepika, Turniansky, Rashi, Green, Erin M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593661/
https://www.ncbi.nlm.nih.gov/pubmed/34816128
http://dx.doi.org/10.1016/j.xpro.2021.100945

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593661/
https://www.ncbi.nlm.nih.gov/pubmed/34816128
http://dx.doi.org/10.1016/j.xpro.2021.100945

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