Cargando…

Synthesis of modified nucleotide polymers by the poly(U) polymerase Cid1: application to direct RNA sequencing on nanopores

Understanding transcriptomes requires documenting the structures, modifications, and abundances of RNAs as well as their proximity to other molecules. The methods that make this possible depend critically on enzymes (including mutant derivatives) that act on nucleic acids for capturing and sequencin...

Descripción completa

Detalles Bibliográficos
Autores principales: Vo, Jenny Mai, Mulroney, Logan, Quick-Cleveland, Jen, Jain, Miten, Akeson, Mark, Ares, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594468/
https://www.ncbi.nlm.nih.gov/pubmed/34446532
http://dx.doi.org/10.1261/rna.078898.121