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Low-bias ncRNA libraries using ordered two-template relay: Serial template jumping by a modified retroelement reverse transcriptase
Selfish, non-long terminal repeat (non-LTR) retroelements and mobile group II introns encode reverse transcriptases (RTs) that can initiate DNA synthesis without substantial base pairing of primer and template. Biochemical characterization of these enzymes has been limited by recombinant expression...
Autores principales: | Upton, Heather E., Ferguson, Lucas, Temoche-Diaz, Morayma M., Liu, Xiao-Man, Pimentel, Sydney C., Ingolia, Nicholas T., Schekman, Randy, Collins, Kathleen |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594491/ https://www.ncbi.nlm.nih.gov/pubmed/34649994 http://dx.doi.org/10.1073/pnas.2107900118 |
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