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Low-bias ncRNA libraries using ordered two-template relay: Serial template jumping by a modified retroelement reverse transcriptase

Selfish, non-long terminal repeat (non-LTR) retroelements and mobile group II introns encode reverse transcriptases (RTs) that can initiate DNA synthesis without substantial base pairing of primer and template. Biochemical characterization of these enzymes has been limited by recombinant expression...

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Detalles Bibliográficos
Autores principales: Upton, Heather E., Ferguson, Lucas, Temoche-Diaz, Morayma M., Liu, Xiao-Man, Pimentel, Sydney C., Ingolia, Nicholas T., Schekman, Randy, Collins, Kathleen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594491/
https://www.ncbi.nlm.nih.gov/pubmed/34649994
http://dx.doi.org/10.1073/pnas.2107900118

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