Fluorine NMR Spectroscopy Enables to Quantify the Affinity Between DNA and Proteins in Cell Lysate

The determination of the binding affinity quantifying the interaction between proteins and nucleic acids is of crucial interest in biological and chemical research. Here, we have made use of site‐specific fluorine labeling of the cold shock protein from Bacillus subtilis, BsCspB, enabling to directl...

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Detalles Bibliográficos
Autores principales: Welte, Hannah, Sinn, Pia, Kovermann, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596521/
https://www.ncbi.nlm.nih.gov/pubmed/34390111
http://dx.doi.org/10.1002/cbic.202100304

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596521/
https://www.ncbi.nlm.nih.gov/pubmed/34390111
http://dx.doi.org/10.1002/cbic.202100304

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