CRISPR/Cas9 mediated editing of the Quorn fungus Fusarium venenatum A3/5 by transient expression of Cas9 and sgRNAs targeting endogenous marker gene PKS12

BACKGROUND: Gene editing using CRISPR/Cas9 is a widely used tool for precise gene modification, modulating gene expression and introducing novel proteins, and its use has been reported in various filamentous fungi including the genus Fusarium. The aim of this study was to optimise gene editing effic...

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Detalles Bibliográficos
Autores principales: Wilson, Fiona M., Harrison, Richard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597179/
https://www.ncbi.nlm.nih.gov/pubmed/34789333
http://dx.doi.org/10.1186/s40694-021-00121-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597179/
https://www.ncbi.nlm.nih.gov/pubmed/34789333
http://dx.doi.org/10.1186/s40694-021-00121-8

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