Simple, efficient and open-source CRISPR/Cas9 strategy for multi-site genome editing in Populus tremula × alba

Although the CRISPR/Cas9 system has been successfully used for crop breeding, its application remains limited in forest trees. Here, we describe an efficient gene editing strategy for hybrid poplar, (Populus tremula × alba INRA clone 717-1B4) based on the Golden Gate MoClo cloning. To test the syste...

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Detalles Bibliográficos
Autores principales: Triozzi, Paolo M, Schmidt, Henry W, Dervinis, Christopher, Kirst, Matias, Conde, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597961/
https://www.ncbi.nlm.nih.gov/pubmed/33960379
http://dx.doi.org/10.1093/treephys/tpab066

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597961/
https://www.ncbi.nlm.nih.gov/pubmed/33960379
http://dx.doi.org/10.1093/treephys/tpab066

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