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Simple, efficient and open-source CRISPR/Cas9 strategy for multi-site genome editing in Populus tremula × alba

Although the CRISPR/Cas9 system has been successfully used for crop breeding, its application remains limited in forest trees. Here, we describe an efficient gene editing strategy for hybrid poplar, (Populus tremula × alba INRA clone 717-1B4) based on the Golden Gate MoClo cloning. To test the syste...

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Detalles Bibliográficos
Autores principales:	Triozzi, Paolo M, Schmidt, Henry W, Dervinis, Christopher, Kirst, Matias, Conde, Daniel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Methods Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597961/ https://www.ncbi.nlm.nih.gov/pubmed/33960379 http://dx.doi.org/10.1093/treephys/tpab066

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