A proteomics protocol to identify stimulation-induced binding partners dependent on a specific gene in mammalian cells

Some protein-protein interactions are induced by different kinds of stimulation and are dependent on specific genes. To identify these interaction partners, we present a protocol which utilizes affinity purification of Flag-tagged protein complexes followed by mass-spectrometry-based proteomics to c...

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Detalles Bibliográficos
Autores principales: Zhu, Junji, Xiao, Wuhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599494/
https://www.ncbi.nlm.nih.gov/pubmed/34820639
http://dx.doi.org/10.1016/j.xpro.2021.100962

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599494/
https://www.ncbi.nlm.nih.gov/pubmed/34820639
http://dx.doi.org/10.1016/j.xpro.2021.100962

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