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A proteomics protocol to identify stimulation-induced binding partners dependent on a specific gene in mammalian cells
Some protein-protein interactions are induced by different kinds of stimulation and are dependent on specific genes. To identify these interaction partners, we present a protocol which utilizes affinity purification of Flag-tagged protein complexes followed by mass-spectrometry-based proteomics to c...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599494/ https://www.ncbi.nlm.nih.gov/pubmed/34820639 http://dx.doi.org/10.1016/j.xpro.2021.100962 |