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Isolation, culture, and immunostaining of neonatal rat ventricular myocytes

Isolation and culture of ventricular cardiomyocytes from neonatal rats (NRVMs) is a powerful model to study neonatal cardiac development, cell cycle regulation, and cardiac physiology and pathology in vitro. Here, we present our modified enzymatic digestion protocol followed by two-step discontinuou...

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Detalles Bibliográficos
Autores principales: Pereira, Ana Helena Macedo, Cardoso, Alisson Campos, Franchini, Kleber Gomes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599495/
https://www.ncbi.nlm.nih.gov/pubmed/34820638
http://dx.doi.org/10.1016/j.xpro.2021.100950