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Isolation, culture, and immunostaining of neonatal rat ventricular myocytes
Isolation and culture of ventricular cardiomyocytes from neonatal rats (NRVMs) is a powerful model to study neonatal cardiac development, cell cycle regulation, and cardiac physiology and pathology in vitro. Here, we present our modified enzymatic digestion protocol followed by two-step discontinuou...
Autores principales: | Pereira, Ana Helena Macedo, Cardoso, Alisson Campos, Franchini, Kleber Gomes |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599495/ https://www.ncbi.nlm.nih.gov/pubmed/34820638 http://dx.doi.org/10.1016/j.xpro.2021.100950 |
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