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uPIC–M: Efficient and Scalable Preparation of Clonal Single Mutant Libraries for High-Throughput Protein Biochemistry

[Image: see text] New high-throughput biochemistry techniques complement selection-based approaches and provide quantitative kinetic and thermodynamic data for thousands of protein variants in parallel. With these advances, library generation rather than data collection has become rate-limiting. Unl...

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Detalles Bibliográficos
Autores principales: Appel, Mason J., Longwell, Scott A., Morri, Maurizio, Neff, Norma, Herschlag, Daniel, Fordyce, Polly M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600632/
https://www.ncbi.nlm.nih.gov/pubmed/34805683
http://dx.doi.org/10.1021/acsomega.1c04180