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uPIC–M: Efficient and Scalable Preparation of Clonal Single Mutant Libraries for High-Throughput Protein Biochemistry
[Image: see text] New high-throughput biochemistry techniques complement selection-based approaches and provide quantitative kinetic and thermodynamic data for thousands of protein variants in parallel. With these advances, library generation rather than data collection has become rate-limiting. Unl...
Autores principales: | Appel, Mason J., Longwell, Scott A., Morri, Maurizio, Neff, Norma, Herschlag, Daniel, Fordyce, Polly M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600632/ https://www.ncbi.nlm.nih.gov/pubmed/34805683 http://dx.doi.org/10.1021/acsomega.1c04180 |
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