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Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
Whole-cell cryo-electron tomography (cryo-ET) is a powerful technology that is used to produce nanometer-level resolution structures of macromolecules present in the cellular context and preserved in a near-native frozen-hydrated state. However, there are challenges associated with culturing and/or...
Autores principales: | Sibert, Bryan S., Kim, Joseph Y., Yang, Jie E., Wright, Elizabeth R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601404/ https://www.ncbi.nlm.nih.gov/pubmed/34570100 http://dx.doi.org/10.3791/62992 |
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