High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome

Gene editing in C. elegans using plasmid-based CRISPR reagents requires microinjection of many animals to produce a single edit. Germline silencing of plasmid-borne Cas9 is a major cause of inefficient editing. Here, we present a set of C. elegans strains that constitutively express Cas9 in the germ...

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Detalles Bibliográficos
Autores principales: Schwartz, Matthew L., Davis, M. Wayne, Rich, Matthew S., Jorgensen, Erik M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601624/
https://www.ncbi.nlm.nih.gov/pubmed/34748534
http://dx.doi.org/10.1371/journal.pgen.1009755

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601624/
https://www.ncbi.nlm.nih.gov/pubmed/34748534
http://dx.doi.org/10.1371/journal.pgen.1009755

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