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Discovery of an F-actin–binding small molecule serving as a fluorescent probe and a scaffold for functional probes
Actin is a ubiquitous cytoskeletal protein, forming a dynamic network that generates mechanical forces in the cell. There is a growing demand for practical and accessible tools for dissecting the role of the actin cytoskeleton in cellular function, and the discovery of a new actin-binding small mole...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604405/ https://www.ncbi.nlm.nih.gov/pubmed/34797716 http://dx.doi.org/10.1126/sciadv.abg8585 |
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author | Takagi, Takeru Ueno, Tasuku Ikawa, Keisuke Asanuma, Daisuke Nomura, Yusuke Uno, Shin-nosuke Komatsu, Toru Kamiya, Mako Hanaoka, Kenjiro Okimura, Chika Iwadate, Yoshiaki Hirose, Kenzo Nagano, Tetsuo Sugimura, Kaoru Urano, Yasuteru |
author_facet | Takagi, Takeru Ueno, Tasuku Ikawa, Keisuke Asanuma, Daisuke Nomura, Yusuke Uno, Shin-nosuke Komatsu, Toru Kamiya, Mako Hanaoka, Kenjiro Okimura, Chika Iwadate, Yoshiaki Hirose, Kenzo Nagano, Tetsuo Sugimura, Kaoru Urano, Yasuteru |
author_sort | Takagi, Takeru |
collection | PubMed |
description | Actin is a ubiquitous cytoskeletal protein, forming a dynamic network that generates mechanical forces in the cell. There is a growing demand for practical and accessible tools for dissecting the role of the actin cytoskeleton in cellular function, and the discovery of a new actin-binding small molecule is an important advance in the field, offering the opportunity to design and synthesize of new class of functional molecules. Here, we found an F-actin–binding small molecule and introduced two powerful tools based on a new class of actin-binding small molecule: One enables visualization of the actin cytoskeleton, including super-resolution imaging, and the other enables highly specific green light–controlled fragmentation of actin filaments, affording unprecedented control of the actin cytoskeleton and its force network in living cells. |
format | Online Article Text |
id | pubmed-8604405 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-86044052021-12-01 Discovery of an F-actin–binding small molecule serving as a fluorescent probe and a scaffold for functional probes Takagi, Takeru Ueno, Tasuku Ikawa, Keisuke Asanuma, Daisuke Nomura, Yusuke Uno, Shin-nosuke Komatsu, Toru Kamiya, Mako Hanaoka, Kenjiro Okimura, Chika Iwadate, Yoshiaki Hirose, Kenzo Nagano, Tetsuo Sugimura, Kaoru Urano, Yasuteru Sci Adv Biomedicine and Life Sciences Actin is a ubiquitous cytoskeletal protein, forming a dynamic network that generates mechanical forces in the cell. There is a growing demand for practical and accessible tools for dissecting the role of the actin cytoskeleton in cellular function, and the discovery of a new actin-binding small molecule is an important advance in the field, offering the opportunity to design and synthesize of new class of functional molecules. Here, we found an F-actin–binding small molecule and introduced two powerful tools based on a new class of actin-binding small molecule: One enables visualization of the actin cytoskeleton, including super-resolution imaging, and the other enables highly specific green light–controlled fragmentation of actin filaments, affording unprecedented control of the actin cytoskeleton and its force network in living cells. American Association for the Advancement of Science 2021-11-19 /pmc/articles/PMC8604405/ /pubmed/34797716 http://dx.doi.org/10.1126/sciadv.abg8585 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Takagi, Takeru Ueno, Tasuku Ikawa, Keisuke Asanuma, Daisuke Nomura, Yusuke Uno, Shin-nosuke Komatsu, Toru Kamiya, Mako Hanaoka, Kenjiro Okimura, Chika Iwadate, Yoshiaki Hirose, Kenzo Nagano, Tetsuo Sugimura, Kaoru Urano, Yasuteru Discovery of an F-actin–binding small molecule serving as a fluorescent probe and a scaffold for functional probes |
title | Discovery of an F-actin–binding small molecule serving as a fluorescent probe and a scaffold for functional probes |
title_full | Discovery of an F-actin–binding small molecule serving as a fluorescent probe and a scaffold for functional probes |
title_fullStr | Discovery of an F-actin–binding small molecule serving as a fluorescent probe and a scaffold for functional probes |
title_full_unstemmed | Discovery of an F-actin–binding small molecule serving as a fluorescent probe and a scaffold for functional probes |
title_short | Discovery of an F-actin–binding small molecule serving as a fluorescent probe and a scaffold for functional probes |
title_sort | discovery of an f-actin–binding small molecule serving as a fluorescent probe and a scaffold for functional probes |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604405/ https://www.ncbi.nlm.nih.gov/pubmed/34797716 http://dx.doi.org/10.1126/sciadv.abg8585 |
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