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Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis

Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse cerebellu...

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Detalles Bibliográficos
Autores principales: Dang, Mai T., Mafra, Fernanda, Haldar, Malay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605103/
https://www.ncbi.nlm.nih.gov/pubmed/34825218
http://dx.doi.org/10.1016/j.xpro.2021.100957