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Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis

Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse cerebellu...

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Detalles Bibliográficos
Autores principales: Dang, Mai T., Mafra, Fernanda, Haldar, Malay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605103/
https://www.ncbi.nlm.nih.gov/pubmed/34825218
http://dx.doi.org/10.1016/j.xpro.2021.100957
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author Dang, Mai T.
Mafra, Fernanda
Haldar, Malay
author_facet Dang, Mai T.
Mafra, Fernanda
Haldar, Malay
author_sort Dang, Mai T.
collection PubMed
description Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse cerebellum without cell sorting to minimize cell damage for scRNA-seq. This protocol includes the procedures for further enrichment of myeloid cells using CD11b+ magnetic beads, followed by the generation of scRNA library and sequencing analysis. For complete details on the use and execution of this protocol, please refer to Dang et al. (2021).
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spelling pubmed-86051032021-11-24 Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis Dang, Mai T. Mafra, Fernanda Haldar, Malay STAR Protoc Protocol Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse cerebellum without cell sorting to minimize cell damage for scRNA-seq. This protocol includes the procedures for further enrichment of myeloid cells using CD11b+ magnetic beads, followed by the generation of scRNA library and sequencing analysis. For complete details on the use and execution of this protocol, please refer to Dang et al. (2021). Elsevier 2021-11-17 /pmc/articles/PMC8605103/ /pubmed/34825218 http://dx.doi.org/10.1016/j.xpro.2021.100957 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Dang, Mai T.
Mafra, Fernanda
Haldar, Malay
Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title_full Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title_fullStr Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title_full_unstemmed Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title_short Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title_sort isolation of myeloid cells from mouse brain tumors for single-cell rna-seq analysis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605103/
https://www.ncbi.nlm.nih.gov/pubmed/34825218
http://dx.doi.org/10.1016/j.xpro.2021.100957
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