Expression and purification of phage T7 ejection proteins for cryo-EM analysis

Bacteriophages of the Podoviridae family densely package their genomes into precursor capsids alongside internal virion proteins called ejection proteins. In phage T7 these proteins (gp14, gp15, and gp16) are ejected into the host envelope forming a DNA-ejectosome for genome delivery. Here, we descr...

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Detalles Bibliográficos
Autores principales: Swanson, Nicholas A., Lokareddy, Ravi K., Li, Fenglin, Hou, Chun-feng, Pavlenok, Mikhail, Niederweis, Michael, Cingolani, Gino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605104/
https://www.ncbi.nlm.nih.gov/pubmed/34825220
http://dx.doi.org/10.1016/j.xpro.2021.100960

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605104/
https://www.ncbi.nlm.nih.gov/pubmed/34825220
http://dx.doi.org/10.1016/j.xpro.2021.100960

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