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Expression and purification of phage T7 ejection proteins for cryo-EM analysis
Bacteriophages of the Podoviridae family densely package their genomes into precursor capsids alongside internal virion proteins called ejection proteins. In phage T7 these proteins (gp14, gp15, and gp16) are ejected into the host envelope forming a DNA-ejectosome for genome delivery. Here, we descr...
Autores principales: | Swanson, Nicholas A., Lokareddy, Ravi K., Li, Fenglin, Hou, Chun-feng, Pavlenok, Mikhail, Niederweis, Michael, Cingolani, Gino |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605104/ https://www.ncbi.nlm.nih.gov/pubmed/34825220 http://dx.doi.org/10.1016/j.xpro.2021.100960 |
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