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Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing

Single-cell RNA sequencing (scRNA-seq) is a powerful tool for enumerating the gene expression dynamics at single-cell resolution. Various organs comprising distinct cellular composition and architecture require unique approaches for highly viable single-cell preparation and reliable sequencing resul...

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Detalles Bibliográficos
Autores principales: Gurumurthy, Rajendra Kumar, Kumar, Naveen, Chumduri, Cindrilla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605478/
https://www.ncbi.nlm.nih.gov/pubmed/34841281
http://dx.doi.org/10.1016/j.xpro.2021.100970