Detection of SARS-CoV-2 spike protein binding to ACE2 in living cells by TR-FRET

The SARS-CoV-2 coronavirus infects human cells through the interaction of the viral envelope spike protein (IPR044366) with the human angiotensin-converting enzyme 2 (ACE2), expressed at the surface of target cells. Here, we describe a detailed protocol to measure the binding of the receptor binding...

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Detalles Bibliográficos
Autores principales: Cecon, Erika, Dam, Julie, Jockers, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606263/
https://www.ncbi.nlm.nih.gov/pubmed/34841271
http://dx.doi.org/10.1016/j.xpro.2021.101024

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606263/
https://www.ncbi.nlm.nih.gov/pubmed/34841271
http://dx.doi.org/10.1016/j.xpro.2021.101024

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