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Phenolic Analysis and In Vitro Biological Activity of Red Wine, Pomace and Grape Seeds Oil Derived from Vitis vinifera L. cv. Montepulciano d’Abruzzo
Grape pomace is commonly considered a waste product of monovarietal red wine production. Methods: HPLC-DAD analysis was performed to determine the polyphenol and flavonoid contents of all the extracts obtained from Montepulciano d’Abruzzo red wine and grape skins whereas, GC-MS was applied to the de...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615145/ https://www.ncbi.nlm.nih.gov/pubmed/34829574 http://dx.doi.org/10.3390/antiox10111704 |
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author | Mollica, Adriano Scioli, Giuseppe Della Valle, Alice Cichelli, Angelo Novellino, Ettore Bauer, Marta Kamysz, Wojciech Llorent-Martínez, Eulogio J. Fernández-de Córdova, Maria Luisa Castillo-López, R. Ak, Gunes Zengin, Gokhan Pieretti, Stefano Stefanucci, Azzurra |
author_facet | Mollica, Adriano Scioli, Giuseppe Della Valle, Alice Cichelli, Angelo Novellino, Ettore Bauer, Marta Kamysz, Wojciech Llorent-Martínez, Eulogio J. Fernández-de Córdova, Maria Luisa Castillo-López, R. Ak, Gunes Zengin, Gokhan Pieretti, Stefano Stefanucci, Azzurra |
author_sort | Mollica, Adriano |
collection | PubMed |
description | Grape pomace is commonly considered a waste product of monovarietal red wine production. Methods: HPLC-DAD analysis was performed to determine the polyphenol and flavonoid contents of all the extracts obtained from Montepulciano d’Abruzzo red wine and grape skins whereas, GC-MS was applied to the determination of fatty acid composition in grape seeds oil. Biological characterization involves antioxidant and antimicrobial assays for all the extracts and seeds oil; Their ability to inhibit α-glucosidase, α-amylase, α-tyrosinase, and ChE enzymes was also detected, together with anti-inflammatory activity on wine, grape skin extracts, and seeds oil by lipoxygenase (5-LOX) and LPS-stimulated macrophage release assays. Data indicate significative polyphenols content (199.31 ± 7.21 mgGAE/g), antioxidant (CUPRAC assay (1036.98 mgTE/g)), enzymatic inhibition (α-tyrosinase: 151.30 ± 1.20 mgKAE/g) and anti-inflammatory activities for wine-organic extract 2, while the antimicrobial activity of grape skin decoction is higher than those reported by wine extracts on three bacterial strains. Interestingly only dealcoholized wine and wine-aqueous extract exerts inhibitory effects on α-glucosidase (20.62 ± 0.23 mmolACAE/g and 19.81 ± 0.03 mmolACAE/g, respectively), while seeds oil is rich in oleic and linoleic acids. These results confirm the strong antioxidant properties of Montepulciano d’Abruzzo grape pomace, suggesting the potential use of this waste product as functional food supplements in the human diet and in cosmeceutics. |
format | Online Article Text |
id | pubmed-8615145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86151452021-11-26 Phenolic Analysis and In Vitro Biological Activity of Red Wine, Pomace and Grape Seeds Oil Derived from Vitis vinifera L. cv. Montepulciano d’Abruzzo Mollica, Adriano Scioli, Giuseppe Della Valle, Alice Cichelli, Angelo Novellino, Ettore Bauer, Marta Kamysz, Wojciech Llorent-Martínez, Eulogio J. Fernández-de Córdova, Maria Luisa Castillo-López, R. Ak, Gunes Zengin, Gokhan Pieretti, Stefano Stefanucci, Azzurra Antioxidants (Basel) Article Grape pomace is commonly considered a waste product of monovarietal red wine production. Methods: HPLC-DAD analysis was performed to determine the polyphenol and flavonoid contents of all the extracts obtained from Montepulciano d’Abruzzo red wine and grape skins whereas, GC-MS was applied to the determination of fatty acid composition in grape seeds oil. Biological characterization involves antioxidant and antimicrobial assays for all the extracts and seeds oil; Their ability to inhibit α-glucosidase, α-amylase, α-tyrosinase, and ChE enzymes was also detected, together with anti-inflammatory activity on wine, grape skin extracts, and seeds oil by lipoxygenase (5-LOX) and LPS-stimulated macrophage release assays. Data indicate significative polyphenols content (199.31 ± 7.21 mgGAE/g), antioxidant (CUPRAC assay (1036.98 mgTE/g)), enzymatic inhibition (α-tyrosinase: 151.30 ± 1.20 mgKAE/g) and anti-inflammatory activities for wine-organic extract 2, while the antimicrobial activity of grape skin decoction is higher than those reported by wine extracts on three bacterial strains. Interestingly only dealcoholized wine and wine-aqueous extract exerts inhibitory effects on α-glucosidase (20.62 ± 0.23 mmolACAE/g and 19.81 ± 0.03 mmolACAE/g, respectively), while seeds oil is rich in oleic and linoleic acids. These results confirm the strong antioxidant properties of Montepulciano d’Abruzzo grape pomace, suggesting the potential use of this waste product as functional food supplements in the human diet and in cosmeceutics. MDPI 2021-10-27 /pmc/articles/PMC8615145/ /pubmed/34829574 http://dx.doi.org/10.3390/antiox10111704 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mollica, Adriano Scioli, Giuseppe Della Valle, Alice Cichelli, Angelo Novellino, Ettore Bauer, Marta Kamysz, Wojciech Llorent-Martínez, Eulogio J. Fernández-de Córdova, Maria Luisa Castillo-López, R. Ak, Gunes Zengin, Gokhan Pieretti, Stefano Stefanucci, Azzurra Phenolic Analysis and In Vitro Biological Activity of Red Wine, Pomace and Grape Seeds Oil Derived from Vitis vinifera L. cv. Montepulciano d’Abruzzo |
title | Phenolic Analysis and In Vitro Biological Activity of Red Wine, Pomace and Grape Seeds Oil Derived from Vitis vinifera L. cv. Montepulciano d’Abruzzo |
title_full | Phenolic Analysis and In Vitro Biological Activity of Red Wine, Pomace and Grape Seeds Oil Derived from Vitis vinifera L. cv. Montepulciano d’Abruzzo |
title_fullStr | Phenolic Analysis and In Vitro Biological Activity of Red Wine, Pomace and Grape Seeds Oil Derived from Vitis vinifera L. cv. Montepulciano d’Abruzzo |
title_full_unstemmed | Phenolic Analysis and In Vitro Biological Activity of Red Wine, Pomace and Grape Seeds Oil Derived from Vitis vinifera L. cv. Montepulciano d’Abruzzo |
title_short | Phenolic Analysis and In Vitro Biological Activity of Red Wine, Pomace and Grape Seeds Oil Derived from Vitis vinifera L. cv. Montepulciano d’Abruzzo |
title_sort | phenolic analysis and in vitro biological activity of red wine, pomace and grape seeds oil derived from vitis vinifera l. cv. montepulciano d’abruzzo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615145/ https://www.ncbi.nlm.nih.gov/pubmed/34829574 http://dx.doi.org/10.3390/antiox10111704 |
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