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Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines
Enzymes of nucleoside and nucleotide metabolism regulate important cellular processes with potential impacts on nucleotide-unrelated parameters. We have used a set of CRISPR/Cas9-modified cell models expressing both, one, or none of the 5′-nucleotidases cN-II and CD73, together with RNA sequencing a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616413/ https://www.ncbi.nlm.nih.gov/pubmed/34831141 http://dx.doi.org/10.3390/cells10112918 |
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author | Cadassou, Octavia Forey, Prescillia Machon, Christelle Petrotto, Edoardo Chettab, Kamel Tozzi, Maria Grazia Guitton, Jérôme Dumontet, Charles Cros-Perrial, Emeline Jordheim, Lars Petter |
author_facet | Cadassou, Octavia Forey, Prescillia Machon, Christelle Petrotto, Edoardo Chettab, Kamel Tozzi, Maria Grazia Guitton, Jérôme Dumontet, Charles Cros-Perrial, Emeline Jordheim, Lars Petter |
author_sort | Cadassou, Octavia |
collection | PubMed |
description | Enzymes of nucleoside and nucleotide metabolism regulate important cellular processes with potential impacts on nucleotide-unrelated parameters. We have used a set of CRISPR/Cas9-modified cell models expressing both, one, or none of the 5′-nucleotidases cN-II and CD73, together with RNA sequencing and targeted metabolomics, to decipher new regulatory roles of these proteins. We observed important transcriptional modifications between models as well as upon exposure to adenosine. Metabolite content varied differently between cell models in response to adenosine exposure but was rather similar in control conditions. Our original cell models allowed us to identify a new unobvious link between proteins in the nucleotide metabolism and other cellular pathways. Further analyses of our models, including additional experiments, could help us to better understand some of the roles played by these enzymes. |
format | Online Article Text |
id | pubmed-8616413 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86164132021-11-26 Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines Cadassou, Octavia Forey, Prescillia Machon, Christelle Petrotto, Edoardo Chettab, Kamel Tozzi, Maria Grazia Guitton, Jérôme Dumontet, Charles Cros-Perrial, Emeline Jordheim, Lars Petter Cells Article Enzymes of nucleoside and nucleotide metabolism regulate important cellular processes with potential impacts on nucleotide-unrelated parameters. We have used a set of CRISPR/Cas9-modified cell models expressing both, one, or none of the 5′-nucleotidases cN-II and CD73, together with RNA sequencing and targeted metabolomics, to decipher new regulatory roles of these proteins. We observed important transcriptional modifications between models as well as upon exposure to adenosine. Metabolite content varied differently between cell models in response to adenosine exposure but was rather similar in control conditions. Our original cell models allowed us to identify a new unobvious link between proteins in the nucleotide metabolism and other cellular pathways. Further analyses of our models, including additional experiments, could help us to better understand some of the roles played by these enzymes. MDPI 2021-10-28 /pmc/articles/PMC8616413/ /pubmed/34831141 http://dx.doi.org/10.3390/cells10112918 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cadassou, Octavia Forey, Prescillia Machon, Christelle Petrotto, Edoardo Chettab, Kamel Tozzi, Maria Grazia Guitton, Jérôme Dumontet, Charles Cros-Perrial, Emeline Jordheim, Lars Petter Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines |
title | Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines |
title_full | Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines |
title_fullStr | Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines |
title_full_unstemmed | Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines |
title_short | Transcriptional and Metabolic Investigation in 5′-Nucleotidase Deficient Cancer Cell Lines |
title_sort | transcriptional and metabolic investigation in 5′-nucleotidase deficient cancer cell lines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616413/ https://www.ncbi.nlm.nih.gov/pubmed/34831141 http://dx.doi.org/10.3390/cells10112918 |
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