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Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations

The quality control of probiotic products is the focus of numerous organizations worldwide. Several studies have highlighted the poor microbiological quality of many commercial probiotic formulations in terms of the identity of the contained microorganisms, viability, and purity, thus precluding the...

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Autores principales: Mazzantini, Diletta, Celandroni, Francesco, Calvigioni, Marco, Panattoni, Adelaide, Labella, Roberto, Ghelardi, Emilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617924/
https://www.ncbi.nlm.nih.gov/pubmed/34829062
http://dx.doi.org/10.3390/foods10112781
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author Mazzantini, Diletta
Celandroni, Francesco
Calvigioni, Marco
Panattoni, Adelaide
Labella, Roberto
Ghelardi, Emilia
author_facet Mazzantini, Diletta
Celandroni, Francesco
Calvigioni, Marco
Panattoni, Adelaide
Labella, Roberto
Ghelardi, Emilia
author_sort Mazzantini, Diletta
collection PubMed
description The quality control of probiotic products is the focus of numerous organizations worldwide. Several studies have highlighted the poor microbiological quality of many commercial probiotic formulations in terms of the identity of the contained microorganisms, viability, and purity, thus precluding the expected health benefits and representing a potential health risk for consumers. In this paper, we analyzed the contents of two probiotic formulations, one composed of an encapsulated mixture of lactobacilli and bifidobacteria, and one by a lyophilized yeast. The microorganisms contained in the products were quantified and identified using up-to-date methodologies, such as MALDI-TOF MS and metagenomic analysis. Moreover, as acid and bile tolerance is included among the criteria used to select probiotic microorganisms, in vitro tests were performed to evaluate the behavior of the formulations in conditions mimicking the harsh gastric environment and the intestinal fluids. Our results indicate the high quality of the formulations in terms of the enumeration and identification of the contained organisms, as well as the absence of contaminants. Moreover, both products tolerated the acidic conditions well, with encapsulation providing further protection for the microorganisms. A good tolerance to the simulated artificial intestinal conditions was also evidenced for both preparations.
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spelling pubmed-86179242021-11-27 Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations Mazzantini, Diletta Celandroni, Francesco Calvigioni, Marco Panattoni, Adelaide Labella, Roberto Ghelardi, Emilia Foods Article The quality control of probiotic products is the focus of numerous organizations worldwide. Several studies have highlighted the poor microbiological quality of many commercial probiotic formulations in terms of the identity of the contained microorganisms, viability, and purity, thus precluding the expected health benefits and representing a potential health risk for consumers. In this paper, we analyzed the contents of two probiotic formulations, one composed of an encapsulated mixture of lactobacilli and bifidobacteria, and one by a lyophilized yeast. The microorganisms contained in the products were quantified and identified using up-to-date methodologies, such as MALDI-TOF MS and metagenomic analysis. Moreover, as acid and bile tolerance is included among the criteria used to select probiotic microorganisms, in vitro tests were performed to evaluate the behavior of the formulations in conditions mimicking the harsh gastric environment and the intestinal fluids. Our results indicate the high quality of the formulations in terms of the enumeration and identification of the contained organisms, as well as the absence of contaminants. Moreover, both products tolerated the acidic conditions well, with encapsulation providing further protection for the microorganisms. A good tolerance to the simulated artificial intestinal conditions was also evidenced for both preparations. MDPI 2021-11-12 /pmc/articles/PMC8617924/ /pubmed/34829062 http://dx.doi.org/10.3390/foods10112781 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mazzantini, Diletta
Celandroni, Francesco
Calvigioni, Marco
Panattoni, Adelaide
Labella, Roberto
Ghelardi, Emilia
Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title_full Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title_fullStr Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title_full_unstemmed Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title_short Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title_sort microbiological quality and resistance to an artificial gut environment of two probiotic formulations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617924/
https://www.ncbi.nlm.nih.gov/pubmed/34829062
http://dx.doi.org/10.3390/foods10112781
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