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Analysis of the Anti-Inflammatory Capacity of Bone Broth in a Murine Model of Ulcerative Colitis

Background and Objectives: Nutritional deficiencies are one of the main triggers for the development of gastrointestinal diseases, such as ulcerative colitis (UC). Therefore, the objective of the present work consisted of determining the nutrients present in the bone broth (BB) and evaluating their...

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Autores principales: Mar-Solís, Laura M., Soto-Domínguez, Adolfo, Rodríguez-Tovar, Luis E., Rodríguez-Rocha, Humberto, García-García, Aracely, Aguirre-Arzola, Víctor E., Zamora-Ávila, Diana E., Garza-Arredondo, Aime J., Castillo-Velázquez, Uziel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618064/
https://www.ncbi.nlm.nih.gov/pubmed/34833355
http://dx.doi.org/10.3390/medicina57111138
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author Mar-Solís, Laura M.
Soto-Domínguez, Adolfo
Rodríguez-Tovar, Luis E.
Rodríguez-Rocha, Humberto
García-García, Aracely
Aguirre-Arzola, Víctor E.
Zamora-Ávila, Diana E.
Garza-Arredondo, Aime J.
Castillo-Velázquez, Uziel
author_facet Mar-Solís, Laura M.
Soto-Domínguez, Adolfo
Rodríguez-Tovar, Luis E.
Rodríguez-Rocha, Humberto
García-García, Aracely
Aguirre-Arzola, Víctor E.
Zamora-Ávila, Diana E.
Garza-Arredondo, Aime J.
Castillo-Velázquez, Uziel
author_sort Mar-Solís, Laura M.
collection PubMed
description Background and Objectives: Nutritional deficiencies are one of the main triggers for the development of gastrointestinal diseases, such as ulcerative colitis (UC). Therefore, the objective of the present work consisted of determining the nutrients present in the bone broth (BB) and evaluating their anti-inflammatory properties in a murine model of UC, induced by intrarectal administration of 2, 4, 6-trinitrobenzene sulfonic acid (TNBS), and acetic acid (AcOH). The BB was prepared from the femur of bovine cattle and cooked in distilled water for 8 h at 100 ± 2 °C. Materials and Methods: The BB was administered ad libitum to BALB/c mice for 10 days before the induction of UC. Colon samples were collected for histological analysis and determination of cytokine expression levels by qPCR. Results: It was found that amino acids (AA) are the main nutritional contribution of BB, 54.56% of these correspond to essential AA. The prophylactic administration of BB in the murine model of UC reduced histological damage, decreased the expression of IL-1β (61.12%), IL-6 (94.70%), and TNF-α (68.88%), and increased the expression of INF-γ (177.06%), IL-4 (541.36%), and IL-10 (531.97%). Conclusions: This study shows that BB has anti-inflammatory properties, and its consumption can decrease the symptoms of UC.
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spelling pubmed-86180642021-11-27 Analysis of the Anti-Inflammatory Capacity of Bone Broth in a Murine Model of Ulcerative Colitis Mar-Solís, Laura M. Soto-Domínguez, Adolfo Rodríguez-Tovar, Luis E. Rodríguez-Rocha, Humberto García-García, Aracely Aguirre-Arzola, Víctor E. Zamora-Ávila, Diana E. Garza-Arredondo, Aime J. Castillo-Velázquez, Uziel Medicina (Kaunas) Article Background and Objectives: Nutritional deficiencies are one of the main triggers for the development of gastrointestinal diseases, such as ulcerative colitis (UC). Therefore, the objective of the present work consisted of determining the nutrients present in the bone broth (BB) and evaluating their anti-inflammatory properties in a murine model of UC, induced by intrarectal administration of 2, 4, 6-trinitrobenzene sulfonic acid (TNBS), and acetic acid (AcOH). The BB was prepared from the femur of bovine cattle and cooked in distilled water for 8 h at 100 ± 2 °C. Materials and Methods: The BB was administered ad libitum to BALB/c mice for 10 days before the induction of UC. Colon samples were collected for histological analysis and determination of cytokine expression levels by qPCR. Results: It was found that amino acids (AA) are the main nutritional contribution of BB, 54.56% of these correspond to essential AA. The prophylactic administration of BB in the murine model of UC reduced histological damage, decreased the expression of IL-1β (61.12%), IL-6 (94.70%), and TNF-α (68.88%), and increased the expression of INF-γ (177.06%), IL-4 (541.36%), and IL-10 (531.97%). Conclusions: This study shows that BB has anti-inflammatory properties, and its consumption can decrease the symptoms of UC. MDPI 2021-10-20 /pmc/articles/PMC8618064/ /pubmed/34833355 http://dx.doi.org/10.3390/medicina57111138 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mar-Solís, Laura M.
Soto-Domínguez, Adolfo
Rodríguez-Tovar, Luis E.
Rodríguez-Rocha, Humberto
García-García, Aracely
Aguirre-Arzola, Víctor E.
Zamora-Ávila, Diana E.
Garza-Arredondo, Aime J.
Castillo-Velázquez, Uziel
Analysis of the Anti-Inflammatory Capacity of Bone Broth in a Murine Model of Ulcerative Colitis
title Analysis of the Anti-Inflammatory Capacity of Bone Broth in a Murine Model of Ulcerative Colitis
title_full Analysis of the Anti-Inflammatory Capacity of Bone Broth in a Murine Model of Ulcerative Colitis
title_fullStr Analysis of the Anti-Inflammatory Capacity of Bone Broth in a Murine Model of Ulcerative Colitis
title_full_unstemmed Analysis of the Anti-Inflammatory Capacity of Bone Broth in a Murine Model of Ulcerative Colitis
title_short Analysis of the Anti-Inflammatory Capacity of Bone Broth in a Murine Model of Ulcerative Colitis
title_sort analysis of the anti-inflammatory capacity of bone broth in a murine model of ulcerative colitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618064/
https://www.ncbi.nlm.nih.gov/pubmed/34833355
http://dx.doi.org/10.3390/medicina57111138
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