Real-Time PCR Assay for Rapid and Simultaneous Detection of vanA and vanB Genes in Clinical Strains

Here, we develop a robust and sensitive real-time PCR assay which allows the simultaneous detection of vanA and vanB genes using common primers. The system was designed using the Primer3 online software. The specificity of primers and probes was first checked by in silico PCR and by BlastN analysis....

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Detalles Bibliográficos
Autores principales: Zerrouki, Hanane, Rebiahi, Sid-Ahmed, Hadjadj, Linda, Rolain, Jean-Marc, Diene, Seydina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618848/
https://www.ncbi.nlm.nih.gov/pubmed/34829428
http://dx.doi.org/10.3390/diagnostics11112081

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618848/
https://www.ncbi.nlm.nih.gov/pubmed/34829428
http://dx.doi.org/10.3390/diagnostics11112081

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