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High-Throughput and Accurate Determination of Transgene Copy Number and Zygosity in Transgenic Maize: From DNA Extraction to Data Analysis

It is vital to develop high-throughput methods to determine transgene copy numbers initially and zygosity during subsequent breeding. In this study, the target sequence of the previously reported endogenous reference gene hmg was analyzed using 633 maize inbred lines, and two SNPs were observed. The...

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Detalles Bibliográficos
Autores principales: Liu, Fang, Cheng, Jinkui, Liu, Xuhua, Wang, Xi-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619409/
https://www.ncbi.nlm.nih.gov/pubmed/34830369
http://dx.doi.org/10.3390/ijms222212487