Cargando…

Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy

Hyaluronic acid, together with collagen, vitamins or plant extracts, is a part of many cosmetic and food preparations. For example, this polysaccharide is used in formulation of many food supplements due to its protective effects on human health. In this work, the screening of the chemical compositi...

Descripción completa

Detalles Bibliográficos
Autores principales: Mirzayeva, Tamilla, Čopíková, Jana, Kvasnička, František, Bleha, Roman, Synytsya, Andriy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620353/
https://www.ncbi.nlm.nih.gov/pubmed/34833306
http://dx.doi.org/10.3390/polym13224002
_version_ 1784605199778709504
author Mirzayeva, Tamilla
Čopíková, Jana
Kvasnička, František
Bleha, Roman
Synytsya, Andriy
author_facet Mirzayeva, Tamilla
Čopíková, Jana
Kvasnička, František
Bleha, Roman
Synytsya, Andriy
author_sort Mirzayeva, Tamilla
collection PubMed
description Hyaluronic acid, together with collagen, vitamins or plant extracts, is a part of many cosmetic and food preparations. For example, this polysaccharide is used in formulation of many food supplements due to its protective effects on human health. In this work, the screening of the chemical composition of three chosen dietary supplements (powder, tablets and capsules) containing hyaluronic acid was carried out using Fourier-transform infrared spectroscopy. Because of the low amount of analyte in all these samples, it was isolated or concentrated prior to the analysis using a suitable sequential fractionation protocol. Individual isolation procedures were established for each sample based on their declared composition. Firstly, the major components such as collagen or vitamins were removed to obtain polysaccharide fractions by the enzymatic treatment and/or washing out with the appropriate solvents. In some cases, the water insoluble part was removed from the rest dissolved in water. Then, hyaluronic acid was precipitated with copper(II) cations and thus separated from the other polysaccharides. Finally, the analyte was identified in the enriched fractions by the characteristic vibrational bands. The amount of hyaluronic acid in the purified fractions was determined in three ways: gravimetrically, spectrophotometrically, and using isotachophoresis. The combination of the appropriate preparative and analytical steps led to the successful evaluation of chemical composition, finding and quantification of hyaluronic acid in all the studied samples.
format Online
Article
Text
id pubmed-8620353
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86203532021-11-27 Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy Mirzayeva, Tamilla Čopíková, Jana Kvasnička, František Bleha, Roman Synytsya, Andriy Polymers (Basel) Communication Hyaluronic acid, together with collagen, vitamins or plant extracts, is a part of many cosmetic and food preparations. For example, this polysaccharide is used in formulation of many food supplements due to its protective effects on human health. In this work, the screening of the chemical composition of three chosen dietary supplements (powder, tablets and capsules) containing hyaluronic acid was carried out using Fourier-transform infrared spectroscopy. Because of the low amount of analyte in all these samples, it was isolated or concentrated prior to the analysis using a suitable sequential fractionation protocol. Individual isolation procedures were established for each sample based on their declared composition. Firstly, the major components such as collagen or vitamins were removed to obtain polysaccharide fractions by the enzymatic treatment and/or washing out with the appropriate solvents. In some cases, the water insoluble part was removed from the rest dissolved in water. Then, hyaluronic acid was precipitated with copper(II) cations and thus separated from the other polysaccharides. Finally, the analyte was identified in the enriched fractions by the characteristic vibrational bands. The amount of hyaluronic acid in the purified fractions was determined in three ways: gravimetrically, spectrophotometrically, and using isotachophoresis. The combination of the appropriate preparative and analytical steps led to the successful evaluation of chemical composition, finding and quantification of hyaluronic acid in all the studied samples. MDPI 2021-11-19 /pmc/articles/PMC8620353/ /pubmed/34833306 http://dx.doi.org/10.3390/polym13224002 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Mirzayeva, Tamilla
Čopíková, Jana
Kvasnička, František
Bleha, Roman
Synytsya, Andriy
Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title_full Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title_fullStr Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title_full_unstemmed Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title_short Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title_sort screening of the chemical composition and identification of hyaluronic acid in food supplements by fractionation and fourier-transform infrared spectroscopy
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620353/
https://www.ncbi.nlm.nih.gov/pubmed/34833306
http://dx.doi.org/10.3390/polym13224002
work_keys_str_mv AT mirzayevatamilla screeningofthechemicalcompositionandidentificationofhyaluronicacidinfoodsupplementsbyfractionationandfouriertransforminfraredspectroscopy
AT copikovajana screeningofthechemicalcompositionandidentificationofhyaluronicacidinfoodsupplementsbyfractionationandfouriertransforminfraredspectroscopy
AT kvasnickafrantisek screeningofthechemicalcompositionandidentificationofhyaluronicacidinfoodsupplementsbyfractionationandfouriertransforminfraredspectroscopy
AT bleharoman screeningofthechemicalcompositionandidentificationofhyaluronicacidinfoodsupplementsbyfractionationandfouriertransforminfraredspectroscopy
AT synytsyaandriy screeningofthechemicalcompositionandidentificationofhyaluronicacidinfoodsupplementsbyfractionationandfouriertransforminfraredspectroscopy