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Grifola frondosa (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in C. elegans through the DAF-16/FOXO and SKN-1/NRF2 Signalling Pathways

In recent years, food ingredients rich in bioactive compounds have emerged as candidates to prevent excess adiposity and other metabolic complications characteristic of obesity, such as low-grade inflammation and oxidative status. Among them, fungi have gained popularity for their high polysaccharid...

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Autores principales: Aranaz, Paula, Peña, Adriana, Vettorazzi, Ariane, Fabra, María José, Martínez-Abad, Antonio, López-Rubio, Amparo, Pera, Joan, Parladé, Javier, Castellari, Massimo, Milagro, Fermín I., González-Navarro, Carlos J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620745/
https://www.ncbi.nlm.nih.gov/pubmed/34836223
http://dx.doi.org/10.3390/nu13113968
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author Aranaz, Paula
Peña, Adriana
Vettorazzi, Ariane
Fabra, María José
Martínez-Abad, Antonio
López-Rubio, Amparo
Pera, Joan
Parladé, Javier
Castellari, Massimo
Milagro, Fermín I.
González-Navarro, Carlos J.
author_facet Aranaz, Paula
Peña, Adriana
Vettorazzi, Ariane
Fabra, María José
Martínez-Abad, Antonio
López-Rubio, Amparo
Pera, Joan
Parladé, Javier
Castellari, Massimo
Milagro, Fermín I.
González-Navarro, Carlos J.
author_sort Aranaz, Paula
collection PubMed
description In recent years, food ingredients rich in bioactive compounds have emerged as candidates to prevent excess adiposity and other metabolic complications characteristic of obesity, such as low-grade inflammation and oxidative status. Among them, fungi have gained popularity for their high polysaccharide content and other bioactive components with beneficial activities. Here, we use the C. elegans model to investigate the potential activities of a Grifola frondosa extract (GE), together with the underlying mechanisms of action. Our study revealed that GE represents an important source of polysaccharides and phenolic compounds with in vitro antioxidant activity. Treatment with our GE extract, which was found to be nongenotoxic through a SOS/umu test, significantly reduced the fat content of C. elegans, decreased the production of intracellular ROS and aging–lipofuscin pigment, and increased the lifespan of nematodes. Gene expression and mutant analyses demonstrated that the in vivo anti-obesity and antioxidant activities of GE were mediated through the daf-2/daf-16 and skn-1/nrf-2 signalling pathways, respectively. Taken together, our results suggest that our GE extract could be considered a potential functional ingredient for the prevention of obesity-related disturbances.
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spelling pubmed-86207452021-11-27 Grifola frondosa (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in C. elegans through the DAF-16/FOXO and SKN-1/NRF2 Signalling Pathways Aranaz, Paula Peña, Adriana Vettorazzi, Ariane Fabra, María José Martínez-Abad, Antonio López-Rubio, Amparo Pera, Joan Parladé, Javier Castellari, Massimo Milagro, Fermín I. González-Navarro, Carlos J. Nutrients Article In recent years, food ingredients rich in bioactive compounds have emerged as candidates to prevent excess adiposity and other metabolic complications characteristic of obesity, such as low-grade inflammation and oxidative status. Among them, fungi have gained popularity for their high polysaccharide content and other bioactive components with beneficial activities. Here, we use the C. elegans model to investigate the potential activities of a Grifola frondosa extract (GE), together with the underlying mechanisms of action. Our study revealed that GE represents an important source of polysaccharides and phenolic compounds with in vitro antioxidant activity. Treatment with our GE extract, which was found to be nongenotoxic through a SOS/umu test, significantly reduced the fat content of C. elegans, decreased the production of intracellular ROS and aging–lipofuscin pigment, and increased the lifespan of nematodes. Gene expression and mutant analyses demonstrated that the in vivo anti-obesity and antioxidant activities of GE were mediated through the daf-2/daf-16 and skn-1/nrf-2 signalling pathways, respectively. Taken together, our results suggest that our GE extract could be considered a potential functional ingredient for the prevention of obesity-related disturbances. MDPI 2021-11-07 /pmc/articles/PMC8620745/ /pubmed/34836223 http://dx.doi.org/10.3390/nu13113968 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aranaz, Paula
Peña, Adriana
Vettorazzi, Ariane
Fabra, María José
Martínez-Abad, Antonio
López-Rubio, Amparo
Pera, Joan
Parladé, Javier
Castellari, Massimo
Milagro, Fermín I.
González-Navarro, Carlos J.
Grifola frondosa (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in C. elegans through the DAF-16/FOXO and SKN-1/NRF2 Signalling Pathways
title Grifola frondosa (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in C. elegans through the DAF-16/FOXO and SKN-1/NRF2 Signalling Pathways
title_full Grifola frondosa (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in C. elegans through the DAF-16/FOXO and SKN-1/NRF2 Signalling Pathways
title_fullStr Grifola frondosa (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in C. elegans through the DAF-16/FOXO and SKN-1/NRF2 Signalling Pathways
title_full_unstemmed Grifola frondosa (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in C. elegans through the DAF-16/FOXO and SKN-1/NRF2 Signalling Pathways
title_short Grifola frondosa (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in C. elegans through the DAF-16/FOXO and SKN-1/NRF2 Signalling Pathways
title_sort grifola frondosa (maitake) extract reduces fat accumulation and improves health span in c. elegans through the daf-16/foxo and skn-1/nrf2 signalling pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620745/
https://www.ncbi.nlm.nih.gov/pubmed/34836223
http://dx.doi.org/10.3390/nu13113968
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