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Mycobiota and Mycotoxin Contamination of Traditional and Industrial Dry-Fermented Sausage Kulen
The aim of this study was to identify and compare surface mycobiota of traditional and industrial Croatian dry-fermented sausage Kulen, especially toxicogenic species, and to detect contamination with mycotoxins recognized as the most important for meat products. Identification of mould species was...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622551/ https://www.ncbi.nlm.nih.gov/pubmed/34822582 http://dx.doi.org/10.3390/toxins13110798 |
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author | Lešić, Tina Zadravec, Manuela Zdolec, Nevijo Vulić, Ana Perković, Irena Škrivanko, Mario Kudumija, Nina Jakopović, Željko Pleadin, Jelka |
author_facet | Lešić, Tina Zadravec, Manuela Zdolec, Nevijo Vulić, Ana Perković, Irena Škrivanko, Mario Kudumija, Nina Jakopović, Željko Pleadin, Jelka |
author_sort | Lešić, Tina |
collection | PubMed |
description | The aim of this study was to identify and compare surface mycobiota of traditional and industrial Croatian dry-fermented sausage Kulen, especially toxicogenic species, and to detect contamination with mycotoxins recognized as the most important for meat products. Identification of mould species was performed by sequence analysis of beta- tubulin and calmodulin gene, while the determination of mycotoxins aflatoxin B(1) (AFB(1)), ochratoxin A (OTA), and cyclopiazonic acid (CPA) was carried out using the LC-MS/MS (liquid chromatography-tandem mass spectrometry) method. The results showed a significantly higher number of mould isolates and greater species (including of those mycotoxigenic) diversity in traditional Kulen samples in comparison with the industrial ones. P. commune, as a potential CPA-producer, was the most represented in traditional Kulen (19.0%), followed by P. solitum (16.6%), which was the most represented in industrial Kulen samples (23.8%). The results also showed that 69% of the traditional sausage samples were contaminated with either CPA or OTA in concentrations of up to 13.35 µg/kg and 6.95 µg/kg, respectively, while in the industrial samples only OTA was detected (in a single sample in the concentration of 0.42 µg/kg). Mycotoxin AFB(1) and its producers were not detected in any of the analysed samples (<LOD). |
format | Online Article Text |
id | pubmed-8622551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86225512021-11-27 Mycobiota and Mycotoxin Contamination of Traditional and Industrial Dry-Fermented Sausage Kulen Lešić, Tina Zadravec, Manuela Zdolec, Nevijo Vulić, Ana Perković, Irena Škrivanko, Mario Kudumija, Nina Jakopović, Željko Pleadin, Jelka Toxins (Basel) Article The aim of this study was to identify and compare surface mycobiota of traditional and industrial Croatian dry-fermented sausage Kulen, especially toxicogenic species, and to detect contamination with mycotoxins recognized as the most important for meat products. Identification of mould species was performed by sequence analysis of beta- tubulin and calmodulin gene, while the determination of mycotoxins aflatoxin B(1) (AFB(1)), ochratoxin A (OTA), and cyclopiazonic acid (CPA) was carried out using the LC-MS/MS (liquid chromatography-tandem mass spectrometry) method. The results showed a significantly higher number of mould isolates and greater species (including of those mycotoxigenic) diversity in traditional Kulen samples in comparison with the industrial ones. P. commune, as a potential CPA-producer, was the most represented in traditional Kulen (19.0%), followed by P. solitum (16.6%), which was the most represented in industrial Kulen samples (23.8%). The results also showed that 69% of the traditional sausage samples were contaminated with either CPA or OTA in concentrations of up to 13.35 µg/kg and 6.95 µg/kg, respectively, while in the industrial samples only OTA was detected (in a single sample in the concentration of 0.42 µg/kg). Mycotoxin AFB(1) and its producers were not detected in any of the analysed samples (<LOD). MDPI 2021-11-12 /pmc/articles/PMC8622551/ /pubmed/34822582 http://dx.doi.org/10.3390/toxins13110798 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lešić, Tina Zadravec, Manuela Zdolec, Nevijo Vulić, Ana Perković, Irena Škrivanko, Mario Kudumija, Nina Jakopović, Željko Pleadin, Jelka Mycobiota and Mycotoxin Contamination of Traditional and Industrial Dry-Fermented Sausage Kulen |
title | Mycobiota and Mycotoxin Contamination of Traditional and Industrial Dry-Fermented Sausage Kulen |
title_full | Mycobiota and Mycotoxin Contamination of Traditional and Industrial Dry-Fermented Sausage Kulen |
title_fullStr | Mycobiota and Mycotoxin Contamination of Traditional and Industrial Dry-Fermented Sausage Kulen |
title_full_unstemmed | Mycobiota and Mycotoxin Contamination of Traditional and Industrial Dry-Fermented Sausage Kulen |
title_short | Mycobiota and Mycotoxin Contamination of Traditional and Industrial Dry-Fermented Sausage Kulen |
title_sort | mycobiota and mycotoxin contamination of traditional and industrial dry-fermented sausage kulen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622551/ https://www.ncbi.nlm.nih.gov/pubmed/34822582 http://dx.doi.org/10.3390/toxins13110798 |
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