Construction of Multiple Guide RNAs in CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case Study for Targeting the FAD2 and FATB Multigene in Soybean

CRISPR/Cas9 is a commonly used technique in reverse-genetics research to knock out a gene of interest. However, when targeting a multigene family or multiple genes, it is necessary to construct a vector with multiple single guide RNAs (sgRNAs) that can navigate the Cas9 protein to the target site. I...

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Detalles Bibliográficos
Autores principales: Kim, Won-Nyeong, Kim, Hye-Jeong, Chung, Young-Soo, Kim, Hyun-Uk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Study Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622832/
https://www.ncbi.nlm.nih.gov/pubmed/34834905
http://dx.doi.org/10.3390/plants10112542

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622832/
https://www.ncbi.nlm.nih.gov/pubmed/34834905
http://dx.doi.org/10.3390/plants10112542

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