Agrobacterium tumefaciens-Mediated Transformation of NHEJ Mutant Aspergillus nidulans Conidia: An Efficient Tool for Targeted Gene Recombination Using Selectable Nutritional Markers

Protoplast transformation for the introduction of recombinant DNA into Aspergillus nidulans is technically demanding and dependant on the availability and batch variability of commercial enzyme preparations. Given the success of Agrobacterium tumefaciens-mediated transformation (ATMT) in diverse pat...

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Detalles Bibliográficos
Autores principales: Casado-del Castillo, Virginia, MacCabe, Andrew P., Orejas, Margarita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623315/
https://www.ncbi.nlm.nih.gov/pubmed/34829246
http://dx.doi.org/10.3390/jof7110961

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623315/
https://www.ncbi.nlm.nih.gov/pubmed/34829246
http://dx.doi.org/10.3390/jof7110961

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