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Quantitative correlative microscopy reveals the ultrastructural distribution of endogenous endosomal proteins

The key endosomal regulators Rab5, EEA1, and APPL1 are frequently applied in fluorescence microscopy to mark early endosomes, whereas Rab7 is used as a marker for late endosomes and lysosomes. However, endogenous levels of these proteins localize poorly in immuno-EM, and systematic studies on their...

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Detalles Bibliográficos
Autores principales: van der Beek, Jan, de Heus, Cecilia, Liv, Nalan, Klumperman, Judith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624803/
https://www.ncbi.nlm.nih.gov/pubmed/34817533
http://dx.doi.org/10.1083/jcb.202106044